What is the catalog number for MPO ELISA Kit (Human)?

The catalog number for MPO ELISA Kit (Human) is OKAU00053-1PLATE.

What sizes are available for MPO ELISA Kit (Human)?

MPO ELISA Kit (Human) is available in the following size(s): 1 Plate.

Is MPO ELISA Kit (Human) in stock?

MPO ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Do you ship this product with dry ice?

Some products, including certain cell lines and biologics, require dry ice for transportation, which carries an additional fee shown clearly in your quotation. Dry ice delivery isn’t always possible to every destination due to transit time or local restrictions, so we check feasibility in advance and you must store the goods at the correct temperature the moment they arrive.

Is this product species cross-reactive?

Cross-reactivity data, based on available testing, is listed under the reactivity section of the datasheet. Contact us if you need reactivity confirmed for a species not listed.

Is there an additional fee for hazardous or dry ice shipping of this product?

Yes, if this product requires dry ice or is classed as hazardous for transport, the additional fee will be explicitly shown in your quotation before you confirm the order, so there are no surprise charges later.

MPO ELISA Kit (Human)

Product Specifications

Reproducibility

Sample Myeloperoxidase Conc. (ng/mL) %CV 1 6.26 2.5 2 4.11 2.4 3 2.35 2.3 Sample Myeloperoxidase Conc. (ng/mL) %CV Sample Myeloperoxidase Conc. (ng/mL) %CV 1 6.26 2.5 1 6.26 2.5 2 4.11 2.4 2 4.11 2.4 3 2.35 2.3 3 2.35 2.3 Sample Myeloperoxidase Conc. (ng/mL) %CV 1 5.64 7.8 2 3.74 7.6 3 2.12 11.8 Sample Myeloperoxidase Conc. (ng/mL) %CV Sample Myeloperoxidase Conc. (ng/mL) %CV 1 5.64 7.8 1 5.64 7.8 2 3.74 7.6 2 3.74 7.6 3 2.12 11.8 3 2.12 11.8

CAS Number

7732-18-5

Reactivity

Human

Type

ELISA Kit

Applications

Enzyme-linked immunosorbent assay

Assay Principle

The human Myeloperoxidase (MPO) EIA kit is designed to quantitatively measure MPO present in a variety of samples and tissue culture media. Please read the complete kit insert before performing this assay. A myeloperoxidase standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with a monoclonal antibody to capture MPO present in the sample. After a 60 minute incubation, the plate is washed. A peroxidase conjugated MPO antibody is added and the plate is again incubated for 60 minutes and washed. Substrate is then added to the plate, which reacts with the bound MPO conjugated antibody. After a third incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of myeloperoxidase in the sample is calculated, after making suitable correction for dilution, using software available with most plate readers.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Platelet-Poor Heparin Plasma, Saliva, Urine and Tissue Culture Media

Sensitivity

0.068 ng/mL

Reconstitution

2°C to 8°C

Components

Component Quantity Mouse anti-Myeloperoxidase Clear Coated 96 Well Plate 1 Plate Human Myeloperoxidase Standard 70 uL Myeloperoxidase Conjugate 5 mL Assay Buffer Concentrate 28 mL Wash Buffer Concentrate 30 mL TMB Substrate 11 mL Stop Solution 5 mL Plate Sealer 2 each Component Quantity Component Quantity Mouse anti-Myeloperoxidase Clear Coated 96 Well Plate 1 Plate Mouse anti-Myeloperoxidase Clear Coated 96 Well Plate 1 Plate Human Myeloperoxidase Standard 70 uL Human Myeloperoxidase Standard 70 uL Myeloperoxidase Conjugate 5 mL Myeloperoxidase Conjugate 5 mL Assay Buffer Concentrate 28 mL Assay Buffer Concentrate 28 mL Wash Buffer Concentrate 30 mL Wash Buffer Concentrate 30 mL TMB Substrate 11 mL TMB Substrate 11 mL Stop Solution 5 mL Stop Solution 5 mL Plate Sealer 2 each Plate Sealer 2 each