T3 ELISA Kit

Sample Triiodothyronine Conc. (pg/mL) %CV 1 629.5 6.6 2 1,169.3 5.5 3 541.6 6.7 Sample Triiodothyronine Conc. (pg/mL) %CV Sample Triiodothyronine Conc. (pg/mL) %CV 1 629.5 6.6 1 629.5 6.6 2 1,169.3 5.5 2 1,169.3 5.5 3 541.6 6.7 3 541.6 6.7 Sample Triiodothyronine Conc. (pg/mL) %CV 1 682.4 11.6 2 1,193.8 14.0 3 587.7 14.7 Sample Triiodothyronine Conc. (pg/mL) %CV Sample Triiodothyronine Conc. (pg/mL) %CV 1 682.4 11.6 1 682.4 11.6 2 1,193.8 14.0 2 1,193.8 14.0 3 587.7 14.7 3 587.7 14.7

The DetectX® Triiodothyronine (T3) Enzyme Immunoassay kit is designed to quantitatively measure T3 present in extracted serum and plasma, urine, extracted dried fecal samples, and tissue culture media samples. Please read the complete kit insert before performing this assay. This kit measures total T3 in extracted serum and plasma and in extracted fecal samples. A T3 stock solution is provided to generate standard curves for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A T3-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a sheep antibody to T3 to each well. After a two hour incubation the plate is washed and substrate is added. The substrate reacts with the bound T3-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the T3 in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Triiodothyronine (T3) Standard 70 uL or 350 uL DetectX® Triiodothyronine (T3) Antibody 3 mL or 13 mL DetectX® Triiodothyronine (T3) Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Coated Clear 96 Well Plates 1 or 5 Each Triiodothyronine (T3) Standard 70 uL or 350 uL Triiodothyronine (T3) Standard 70 uL or 350 uL DetectX® Triiodothyronine (T3) Antibody 3 mL or 13 mL DetectX® Triiodothyronine (T3) Antibody 3 mL or 13 mL DetectX® Triiodothyronine (T3) Conjugate 3 mL or 13 mL DetectX® Triiodothyronine (T3) Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each