Aldosterone ELISA Kit

Sample Aldosterone Conc. (pg/mL) %CV 1 116.0 5.8 2 21.8 10.1 3 6.33 11.4 Sample Aldosterone Conc. (pg/mL) %CV Sample Aldosterone Conc. (pg/mL) %CV 1 116.0 5.8 1 116.0 5.8 2 21.8 10.1 2 21.8 10.1 3 6.33 11.4 3 6.33 11.4 Sample Aldosterone Conc. (pg/mL) %CV 1 120.2 8.7 2 23.9 13.7 3 6.89 20.0 Sample Aldosterone Conc. (pg/mL) %CV Sample Aldosterone Conc. (pg/mL) %CV 1 120.2 8.7 1 120.2 8.7 2 23.9 13.7 2 23.9 13.7 3 6.89 20.0 3 6.89 20.0

The DetectX® Aldosterone Chemiluminescent Immunoassay (CLIA) kit is designed to quantitatively measure Aldosterone present in extracted serum and plasma, or in saliva, urine, extracted dried fecal samples, and tissue culture media samples. Please read the complete kit insert before performing this assay. This kit measures total aldosterone in extracted serum or plasma and fecal samples. An aldosterone stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a white microtiter plate coated with an antibody to capture sheep antibodies. An aldosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to aldosterone to each well. After an overnight incubation at 4C, the plate is washed and substrate is added. The chemiluminescent substrate reacts with the bound aldosterone-peroxidase conjugate to generate light. The generated luminescent signal is detected in a microtiter plate luminometer or multmode reader capable of measuring luminescence. The concentration of the aldosterone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Coated White 96 Well Plate 1 or 5 Each Aldosterone Standard 125 uL or 625 uL DetectX® Aldosterone CLIA Antibody 3 mL or 13 mL DetectX® Aldosterone CLIA Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL Substrate Solution A 6 mL or 28 mL Substrate Solution B 6 mL or 28 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated White 96 Well Plate 1 or 5 Each Coated White 96 Well Plate 1 or 5 Each Aldosterone Standard 125 uL or 625 uL Aldosterone Standard 125 uL or 625 uL DetectX® Aldosterone CLIA Antibody 3 mL or 13 mL DetectX® Aldosterone CLIA Antibody 3 mL or 13 mL DetectX® Aldosterone CLIA Conjugate 3 mL or 13 mL DetectX® Aldosterone CLIA Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL Substrate Solution A 6 mL or 28 mL Substrate Solution A 6 mL or 28 mL Substrate Solution B 6 mL or 28 mL Substrate Solution B 6 mL or 28 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each