PGE2 ELISA Kit

Sample Prostaglandin E2 Conc. (pg/mL) %CV 1 11.7 12.3 2 98.6 6.3 3 131.2 4.9 Sample Prostaglandin E2 Conc. (pg/mL) %CV Sample Prostaglandin E2 Conc. (pg/mL) %CV 1 11.7 12.3 1 11.7 12.3 2 98.6 6.3 2 98.6 6.3 3 131.2 4.9 3 131.2 4.9 Sample Prostaglandin E2 Conc. (pg/mL) %CV 1 12.3 8.8 2 100.5 8.1 3 134.7 9.8 Sample Prostaglandin E2 Conc. (pg/mL) %CV Sample Prostaglandin E2 Conc. (pg/mL) %CV 1 12.3 8.8 1 12.3 8.8 2 100.5 8.1 2 100.5 8.1 3 134.7 9.8 3 134.7 9.8

The DetectX® Prostaglandin E2 (PGE2) Immunoassay kit is designed to quantitatively measure PGE2 present in serum, plasma, urine, saliva, cells, tissue, and tissue culture media samples. This EIA kit allows for the widest variations in sample size, sensitivity and assay timing of any PGE2 kit. The protocol variations are outlined on page 5. Please read the complete kit insert before performing this assay. A PGE2 standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse IgG. A PGE2 - peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to PGE2 to each well. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound PGE2 -peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the PGE2 in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Prostaglandin E2 Standard 70 uL or 350 uL DetectX® Prostaglandin E2 Antibody 3 mL or 13 mL DetectX® Prostaglandin E2 Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Coated Clear 96 Well Plates 1 or 5 Each Prostaglandin E2 Standard 70 uL or 350 uL Prostaglandin E2 Standard 70 uL or 350 uL DetectX® Prostaglandin E2 Antibody 3 mL or 13 mL DetectX® Prostaglandin E2 Antibody 3 mL or 13 mL DetectX® Prostaglandin E2 Conjugate 3 mL or 13 mL DetectX® Prostaglandin E2 Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each