AVP ELISA Kit

Sample AVP Conc. (pg/mL) %CV 1 293.1 11.1% 2 43.0 7.4% 3 10.4 9.9% Sample AVP Conc. (pg/mL) %CV Sample AVP Conc. (pg/mL) %CV 1 293.1 11.1% 1 293.1 11.1% 2 43.0 7.4% 2 43.0 7.4% 3 10.4 9.9% 3 10.4 9.9% Sample AVP Conc. (pg/mL) %CV 1 271.1 16.9% 2 39.8 10.4% 3 10.3 16.1% Sample AVP Conc. (pg/mL) %CV Sample AVP Conc. (pg/mL) %CV 1 271.1 16.9% 1 271.1 16.9% 2 39.8 10.4% 2 39.8 10.4% 3 10.3 16.1% 3 10.3 16.1%

The DetectX® Arg8 -Vasopressin (AVP) Chemiluminescent Immunoassay Kit is designed to quantitatively measure AVP present in serum, plasma and tissue culture media samples. Please read the complete kit insert before performing this assay. An AVP standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a white microtiter plate coated with an antibody to capture rabbit antibodies. An AVP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to AVP to each well. After an overnight incubation at 4C the plate is washed and supplied substrate is added. The substrate reacts with the bound AVP-peroxidase conjugate. The intensity of the generated chemiluminescent signal is detected in a microtiter plate reader capable of measuring luminescence. The concentration of the AVP in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Coated White 96 Well Plates 1 or 5 Each Arg-Vasopressin Standard 25 uL or 125 uL DetectX® Arg-Vasopressin Antibody 3 mL or 13 mL DetectX® Arg-Vasopressin Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Extraction Solution 50 mL or 250 mL Wash Buffer Concentrate 30 mL or 125 mL Substrate Solution A 6 mL or 28 mL Substrate Solution B 6 mL or 28 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated White 96 Well Plates 1 or 5 Each Coated White 96 Well Plates 1 or 5 Each Arg-Vasopressin Standard 25 uL or 125 uL Arg-Vasopressin Standard 25 uL or 125 uL DetectX® Arg-Vasopressin Antibody 3 mL or 13 mL DetectX® Arg-Vasopressin Antibody 3 mL or 13 mL DetectX® Arg-Vasopressin Conjugate 3 mL or 13 mL DetectX® Arg-Vasopressin Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Extraction Solution 50 mL or 250 mL Extraction Solution 50 mL or 250 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL Substrate Solution A 6 mL or 28 mL Substrate Solution A 6 mL or 28 mL Substrate Solution B 6 mL or 28 mL Substrate Solution B 6 mL or 28 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each