PRL ELISA Kit

Sample Prolactin Conc. (pg/mL) %CV 1 883.8 2.9 2 290.7 5.7 3 73.1 14.3 Sample Prolactin Conc. (pg/mL) %CV Sample Prolactin Conc. (pg/mL) %CV 1 883.8 2.9 1 883.8 2.9 2 290.7 5.7 2 290.7 5.7 3 73.1 14.3 3 73.1 14.3 Sample Prolactin Conc. (pg/mL) %CV 1 871.6 3.9 2 290.4 8.2 3 59.6 20.2 Sample Prolactin Conc. (pg/mL) %CV Sample Prolactin Conc. (pg/mL) %CV 1 871.6 3.9 1 871.6 3.9 2 290.4 8.2 2 290.4 8.2 3 59.6 20.2 3 59.6 20.2

The DetectX® Prolactin Immunoassay Kit is designed to quantitatively measure prolactin present in biological samples and tissue culture media. The kit has been tested to measure human and elephant serum prolactin. Please check citations to determine if other species have been measured. Please read the complete kit insert before performing this assay. A human prolactin standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with a monoclonal antibody to capture the prolactin present. After a 60 minute incubation, the plate is washed and a peroxidase conjugated prolactin polyclonal antibody is added. The plate is again incubated for 60 minutes and washed. Substrate is then added to the plate, which reacts with the bound prolactin antibody conjugate. After a third incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the prolactin in the sample is calculated, after making suitable correction for dilution, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Clear Coated 96 Well Plate 1 Plate Prolactin Standard 2 Vials DetectX® Prolactin Conjugate 5 mL Assay Buffer Concentrate 28 mL Wash Buffer Concentrate 30 mL TMB Substrate 11 mL Stop Solution 5 mL Plate Sealer 2 each Component Quantity Component Quantity Clear Coated 96 Well Plate 1 Plate Clear Coated 96 Well Plate 1 Plate Prolactin Standard 2 Vials Prolactin Standard 2 Vials DetectX® Prolactin Conjugate 5 mL DetectX® Prolactin Conjugate 5 mL Assay Buffer Concentrate 28 mL Assay Buffer Concentrate 28 mL Wash Buffer Concentrate 30 mL Wash Buffer Concentrate 30 mL TMB Substrate 11 mL TMB Substrate 11 mL Stop Solution 5 mL Stop Solution 5 mL Plate Sealer 2 each Plate Sealer 2 each