E1S ELISA Kit

Sample E1S Conc. (pg/mL) %CV 1 1,051.7 2.8 2 437.7 3.8 3 163.8 6.0 Sample E1S Conc. (pg/mL) %CV Sample E1S Conc. (pg/mL) %CV 1 1,051.7 2.8 1 1,051.7 2.8 2 437.7 3.8 2 437.7 3.8 3 163.8 6.0 3 163.8 6.0 Sample E1S Conc. (pg/mL) %CV 1 1,025.4 8.1 2 459.9 9.4 3 158.0 8.1 Sample E1S Conc. (pg/mL) %CV Sample E1S Conc. (pg/mL) %CV 1 1,025.4 8.1 1 1,025.4 8.1 2 459.9 9.4 2 459.9 9.4 3 158.0 8.1 3 158.0 8.1

The DetectX® Estrone-3-Sulfate (E1S) Immunoassay Kit uses a specifically generated antibody to measure E1S in a variety of matrices, including serum, plasma, urine and fecal samples. The kit will quantitatively measure E1S present in diluted buffer samples and tissue culture media samples. Please read the complete kit insert before performing this assay. An E1S standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An E1S-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to E1S to each well. After a 2 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound E1S-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the E1S in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Estrone-3-Sulfate (E1S) Standard 125 uL or 625 uL DetectX® Estrone-3-Sulfate (E1S) Antibody 3 mL or 13 mL DetectX® Estrone-3-Sulfate (E1S) Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Dissociation Reagent 1 mL or 5 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Coated Clear 96 Well Plates 1 or 5 Each Estrone-3-Sulfate (E1S) Standard 125 uL or 625 uL Estrone-3-Sulfate (E1S) Standard 125 uL or 625 uL DetectX® Estrone-3-Sulfate (E1S) Antibody 3 mL or 13 mL DetectX® Estrone-3-Sulfate (E1S) Antibody 3 mL or 13 mL DetectX® Estrone-3-Sulfate (E1S) Conjugate 3 mL or 13 mL DetectX® Estrone-3-Sulfate (E1S) Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Dissociation Reagent 1 mL or 5 mL Dissociation Reagent 1 mL or 5 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each