E1G ELISA Kit

Sample E1G Conc. (pg/mL) %CV 1 241.1 3.1 2 70.7 3.5 3 39.9 4.7 Sample E1G Conc. (pg/mL) %CV Sample E1G Conc. (pg/mL) %CV 1 241.1 3.1 1 241.1 3.1 2 70.7 3.5 2 70.7 3.5 3 39.9 4.7 3 39.9 4.7 Sample E1G Conc. (pg/mL) %CV 1 252.8 4.7 2 70.7 5.9 3 38.9 6.3 Sample E1G Conc. (pg/mL) %CV Sample E1G Conc. (pg/mL) %CV 1 252.8 4.7 1 252.8 4.7 2 70.7 5.9 2 70.7 5.9 3 38.9 6.3 3 38.9 6.3

The DetectX® Estrone-3-Glucuronide (E1G) Enzyme Immunoassay Kit uses a specifically generated antibody to measure E1G and its metabolites in urine and fecal samples, or in extracted serum and plasma. This kit is not recommended for serum or plasma samples without extraction. The kit will quantitatively measure E1G present in diluted buffer samples and tissue culture media samples. Please read the complete kit insert before performing this assay. An E1G standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. Two formats can be chosen. Using 50 uL of sample or standards provides a standard curve from 1,000 to 15.625 pg/mL. A 100 uL standard and sample volume format will give a standard curve from 250 to 3.906 pg/mL. An E1G-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to E1G to each well. After a 2 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound E1G-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the E1G in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Estrone-3-Glucuronide (E1G) Standard 125 uL or 625 uL DetectX® Estrone-3-Glucuronide (E1G) Antibody 3 mL or 13 mL DetectX® Estrone-3-Glucuronide (E1G) Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Coated Clear 96 Well Plates 1 or 5 Each Estrone-3-Glucuronide (E1G) Standard 125 uL or 625 uL Estrone-3-Glucuronide (E1G) Standard 125 uL or 625 uL DetectX® Estrone-3-Glucuronide (E1G) Antibody 3 mL or 13 mL DetectX® Estrone-3-Glucuronide (E1G) Antibody 3 mL or 13 mL DetectX® Estrone-3-Glucuronide (E1G) Conjugate 3 mL or 13 mL DetectX® Estrone-3-Glucuronide (E1G) Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each