Ceruloplasmin Colorimetric Activity Kit

Sample Ceruloplasmin Activity (mU/mL) %CV 1 468.5 5.0 2 299.7 7.0 Sample Ceruloplasmin Activity (mU/mL) %CV Sample Ceruloplasmin Activity (mU/mL) %CV 1 468.5 5.0 1 468.5 5.0 2 299.7 7.0 2 299.7 7.0 Sample Ceruloplasmin Activity (mU/mL) %CV 1 480.6 9.6 2 285.4 18.8 Sample Ceruloplasmin Activity (mU/mL) %CV Sample Ceruloplasmin Activity (mU/mL) %CV 1 480.6 9.6 1 480.6 9.6 2 285.4 18.8 2 285.4 18.8

The DetectX® Ceruloplasmin Activity Kit is designed to quantitatively measure ceruloplasmin activity in diluted serum and urine samples. Please read the complete kit insert before performing this assay. A human ceruloplasmin standard is provided to generate a standard curve for the assay and all samples should be read off of the standard curve. Samples are diluted in the provided Assay Buffer and added to the wells of a half area clear plate. The reconstituted Ceruloplasmin Substrate is added and the plate is incubated at 30C for 60 minutes. The ceruloplasmin in the standards and samples reacts with the substrate to produce a colored product. The optical density is read at 560 nm. Increasing levels of ceruloplasmin in the samples causes an increase in the fuchsia (pink-purple) product. The activity of the ceruloplasmin in the sample is calculated after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of milliunits (mU) of ceruloplasmin activity per mL. Ceruloplasmin activity is based upon the published determination by G. Curzon and L. Vallet, Biochem. J., 1960, 74:279-287. Due to our optimized reagents and incubation times the optical density generated by 1 U/mL of ceruloplasmin is substantially higher than 0.1 OD.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Clear Half Area 96 well Plates 2 Plates Ceruloplasmin Standard 20 uL Assay Buffer Concentrate 28 mL Ceruloplasmin Colorimetric Substrate 2 Vials Plate Sealers 2 Each Component Quantity Component Quantity Clear Half Area 96 well Plates 2 Plates Clear Half Area 96 well Plates 2 Plates Ceruloplasmin Standard 20 uL Ceruloplasmin Standard 20 uL Assay Buffer Concentrate 28 mL Assay Buffer Concentrate 28 mL Ceruloplasmin Colorimetric Substrate 2 Vials Ceruloplasmin Colorimetric Substrate 2 Vials Plate Sealers 2 Each Plate Sealers 2 Each