Catalase Fluorescent Activity Kit

Sample Catalase Activity (U/mL) %CV 1 2.89 3.2 2 1.15 4.6 3 0.69 5.9 Sample Catalase Activity (U/mL) %CV Sample Catalase Activity (U/mL) %CV 1 2.89 3.2 1 2.89 3.2 2 1.15 4.6 2 1.15 4.6 3 0.69 5.9 3 0.69 5.9 Sample Catalase Activity (U/mL) %CV 1 2.85 7.3 2 1.10 7.8 3 0.63 7.4 Sample Catalase Activity (U/mL) %CV Sample Catalase Activity (U/mL) %CV 1 2.85 7.3 1 2.85 7.3 2 1.10 7.8 2 1.10 7.8 3 0.63 7.4 3 0.63 7.4

The DetectX® Catalase Activity Kit is designed to quantitatively measure catalase activity in a variety of samples. Please read the complete kit insert before performing this assay. A bovine catalase standard is provided to generate a standard curve for the assay and all samples should be read off of the standard curve. Samples are diluted in the provided Assay Buffer and added to the wells of a half area black plate. Hydrogen peroxide is added to each well and the plate incubated at room temperature for 30 minutes. The supplied Fluorescent Detection Reagent is added, followed by diluted horseradish peroxidase and incubated at room temperature for 15 minutes. The HRP reacts with the substrate in the presence of hydrogen peroxide to convert the colorless substrate into a fluorescent product. The fluorescent product is read at 590 nm with excitation at 570 nm. Increasing levels of catalase in the samples causes a decrease in H2O2 concentration and a reduction in fluorescent product. The activity of the catalase in the sample is calculated after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of units of catalase activity per mL.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Black Half Area 96 well Plates 2 Plates Catalog Number X037-2EA Catalase Standard 90 uL Catalog Number C114-90UL Assay Buffer Concentrate 25 mL Catalog Number X106-25ML Hydrogen Peroxide Reagent 5 mL Catalog Number C115-5ML Fluorescent Detection Reagent 5 mL Catalog Number C116-5ML Horseradish Peroxidase Concentrate 60 uL Component Quantity Component Quantity Black Half Area 96 well Plates 2 Plates Catalog Number X037-2EA Black Half Area 96 well Plates 2 Plates Catalog Number X037-2EA Catalase Standard 90 uL Catalog Number C114-90UL Catalase Standard 90 uL Catalog Number C114-90UL Assay Buffer Concentrate 25 mL Catalog Number X106-25ML Assay Buffer Concentrate 25 mL Catalog Number X106-25ML Hydrogen Peroxide Reagent 5 mL Catalog Number C115-5ML Hydrogen Peroxide Reagent 5 mL Catalog Number C115-5ML Fluorescent Detection Reagent 5 mL Catalog Number C116-5ML Fluorescent Detection Reagent 5 mL Catalog Number C116-5ML Horseradish Peroxidase Concentrate 60 uL Horseradish Peroxidase Concentrate 60 uL