Estradiol ELISA Kit

Sample Estradiol Conc. (pg/mL) %CV 1 2,935 3.9 2 881.3 4.0 3 213.9 7.3 Sample Estradiol Conc. (pg/mL) %CV Sample Estradiol Conc. (pg/mL) %CV 1 2,935 3.9 1 2,935 3.9 2 881.3 4.0 2 881.3 4.0 3 213.9 7.3 3 213.9 7.3 Sample Estradiol Conc. (pg/mL) %CV 1 2,797 3.6 2 851.2 7.8 3 216.1 13.8 Sample Estradiol Conc. (pg/mL) %CV Sample Estradiol Conc. (pg/mL) %CV 1 2,797 3.6 1 2,797 3.6 2 851.2 7.8 2 851.2 7.8 3 216.1 13.8 3 216.1 13.8

The DetectX® Estradiol Immunoassay kit uses a specifically generated antibody to measure estradiol and its metabolites in urine and fecal samples. This kit is not recommended for serum, plasma, or saliva samples as the concentration of estradiol in these samples is too low to be measured without significant concentration. The kit will quantitatively measure Estradiol present in reconstituted buffer samples and tissue culture media samples. Please read the complete kit insert before performing this assay. An estradiol standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An estradiol-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to estradiol to each well. After a 2 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound estradiol-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the estradiol in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Estradiol Standard 125 uL or 625 uL DetectX® Estradiol Antibody 3 mL or 13 mL DetectX® Estradiol Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Coated Clear 96 Well Plates 1 or 5 Each Estradiol Standard 125 uL or 625 uL Estradiol Standard 125 uL or 625 uL DetectX® Estradiol Antibody 3 mL or 13 mL DetectX® Estradiol Antibody 3 mL or 13 mL DetectX® Estradiol Conjugate 3 mL or 13 mL DetectX® Estradiol Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each