SOD Colorimetric Activity Kit

Sample SOD Activity (U/mL) %CV 1 0.407 4.6 2 0.726 7.3 3 1.203 16.8 Sample SOD Activity (U/mL) %CV Sample SOD Activity (U/mL) %CV 1 0.407 4.6 1 0.407 4.6 2 0.726 7.3 2 0.726 7.3 3 1.203 16.8 3 1.203 16.8 Sample SOD Activity (U/mL) %CV 1 0.356 10.5 2 0.653 6.1 3 1.277 13.8 Sample SOD Activity (U/mL) %CV Sample SOD Activity (U/mL) %CV 1 0.356 10.5 1 0.356 10.5 2 0.653 6.1 2 0.653 6.1 3 1.277 13.8 3 1.277 13.8

The DetectX® Superoxide Dismutase (SOD) Activity Kit is designed to quantitatively measure SOD activity in a variety of samples. The assay measures all types of SOD activity, including Cu/Zn, Mn, and FeSOD types. Please read the complete kit insert before performing this assay. A bovine erythrocyte SOD standard is provided to generate a standard curve for the assay and all samples should be read off of the standard curve. Samples are diluted in our specially colored Assay Buffer and added to the wells. The Substrate is added followed by Xanthine Oxidase Reagent and incubated at room temperature for 20 minutes. The Xanthine Oxidase generates superoxide in the presence of oxygen, which converts a colorless substrate in the Detection Reagent into a yellow colored product. The colored product is read at 450 nm. Increasing levels of SOD in the samples causes a decrease in superoxide concentration and a reduction in yellow product. The activity of the SOD in the sample is calculated after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of units of SOD activity per mL.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Clear 96 well Half Area Plates 2 Plates Superoxide Dismutase Standard 1 Vial Assay Buffer 50 mL Xanthine Oxidase Buffer 6 mL Xanthine Oxidase Concentrate 225 uL Substrate Diluent 12 mL Substrate Concentrate 1.1 mL Component Quantity Component Quantity Clear 96 well Half Area Plates 2 Plates Clear 96 well Half Area Plates 2 Plates Superoxide Dismutase Standard 1 Vial Superoxide Dismutase Standard 1 Vial Assay Buffer 50 mL Assay Buffer 50 mL Xanthine Oxidase Buffer 6 mL Xanthine Oxidase Buffer 6 mL Xanthine Oxidase Concentrate 225 uL Xanthine Oxidase Concentrate 225 uL Substrate Diluent 12 mL Substrate Diluent 12 mL Substrate Concentrate 1.1 mL Substrate Concentrate 1.1 mL