PGFM ELISA Kit

Sample PGFM Conc. (pg/mL) %CV 1 1,428.9 6.9 2 464.6 7.5 3 217.7 13.2 Sample PGFM Conc. (pg/mL) %CV Sample PGFM Conc. (pg/mL) %CV 1 1,428.9 6.9 1 1,428.9 6.9 2 464.6 7.5 2 464.6 7.5 3 217.7 13.2 3 217.7 13.2 Sample PGFM Conc. (pg/mL) %CV 1 1,485.2 6.8 2 472.2 9.6 3 189.7 12.6 Sample PGFM Conc. (pg/mL) %CV Sample PGFM Conc. (pg/mL) %CV 1 1,485.2 6.8 1 1,485.2 6.8 2 472.2 9.6 2 472.2 9.6 3 189.7 12.6 3 189.7 12.6

The DetectX® 13,14-dihydro-15-keto-PGF2a (PGFM) Immunoassay Kit is a patent pending assay from Dr.Martin Dehnhard at Leibniz Institute for Zoo & Wildlife Research, Berlin, Germany licensed exclusively and designed to quantitatively measure PGFM present in fecal extracts, urine, serum and plasma samples. Please read the complete kit insert before performing this assay. A PGFM standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit IgG. A PGFM-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody highly specific to PGFM to each well. After an hour incubation, the plate is washed and substrate is added. The substrate reacts with the bound PGFM-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the PGFM in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Coated Clear 96 Well Plates 1 or 5 Each PGFM Standard 125 uL or 625 uL DetectX® PGFM Antibody 3 mL or 13 mL DetectX® PGFM Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Coated Clear 96 Well Plates 1 or 5 Each PGFM Standard 125 uL or 625 uL PGFM Standard 125 uL or 625 uL DetectX® PGFM Antibody 3 mL or 13 mL DetectX® PGFM Antibody 3 mL or 13 mL DetectX® PGFM Conjugate 3 mL or 13 mL DetectX® PGFM Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each