OPN ELISA Kit (Human)

Sample Osteopontin Conc. (ng/mL) %CV 1 19.32 1.5 2 9.86 2.6 3 6.75 2.6 Sample Osteopontin Conc. (ng/mL) %CV Sample Osteopontin Conc. (ng/mL) %CV 1 19.32 1.5 1 19.32 1.5 2 9.86 2.6 2 9.86 2.6 3 6.75 2.6 3 6.75 2.6 Sample Osteopontin Conc. (ng/mL) %CV 1 19.17 3.5 2 9.73 5.8 3 6.01 9.5 Sample Osteopontin Conc. (ng/mL) %CV Sample Osteopontin Conc. (ng/mL) %CV 1 19.17 3.5 1 19.17 3.5 2 9.73 5.8 2 9.73 5.8 3 6.01 9.5 3 6.01 9.5

The DetectX® Human Osteopontin Immunoassay Kit is designed to quantitatively measure human Osteopontin present in biological samples and tissue culture media. Please read the complete kit insert before performing this assay. A human Osteopontin standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with a monoclonal antibody to capture the Osteopontin present. After a 60 minute incubation, the plate is washed and a peroxidase conjugated Osteopontin monoclonal antibody is added. The plate is again incubated for 60 minutes and washed. Substrate is then added to the plate, which reacts with the bound Osteopontin Antibody Conjugate. After a third incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the Osteopontin in the sample is calculated, after making suitable correction for dilution, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Clear Coated 96 Well Plate 1 Plate Osteopontin Standard 2 Vials DetectX® Osteopontin Conjugate 5 mL Assay Buffer Concentrate 28 mL Wash Buffer Concentrate 30 mL TMB Substrate 11 mL Stop Solution 5 mL Plate Sealer 1 Each Component Quantity Component Quantity Clear Coated 96 Well Plate 1 Plate Clear Coated 96 Well Plate 1 Plate Osteopontin Standard 2 Vials Osteopontin Standard 2 Vials DetectX® Osteopontin Conjugate 5 mL DetectX® Osteopontin Conjugate 5 mL Assay Buffer Concentrate 28 mL Assay Buffer Concentrate 28 mL Wash Buffer Concentrate 30 mL Wash Buffer Concentrate 30 mL TMB Substrate 11 mL TMB Substrate 11 mL Stop Solution 5 mL Stop Solution 5 mL Plate Sealer 1 Each Plate Sealer 1 Each