Cortisone ELISA Kit

Sample Cortisone Conc. (pg/mL) %CV 1 116.4 13.0 2 414.6 8.6 3 3,320 10.8 Sample Cortisone Conc. (pg/mL) %CV Sample Cortisone Conc. (pg/mL) %CV 1 116.4 13.0 1 116.4 13.0 2 414.6 8.6 2 414.6 8.6 3 3,320 10.8 3 3,320 10.8 Sample Cortisone Conc. (pg/mL) %CV 1 108.1 15.1 2 414.5 9.0 3 3,183 9.8 Sample Cortisone Conc. (pg/mL) %CV Sample Cortisone Conc. (pg/mL) %CV 1 108.1 15.1 1 108.1 15.1 2 414.5 9.0 2 414.5 9.0 3 3,183 9.8 3 3,183 9.8

The DetectX® Cortisone Chemiluminescent Immunoassay Kit is designed to quantitatively measure Cortisone present in extracted dried fecal samples, urine, saliva, plasma, and serum samples. Please read the complete kit insert before performing this assay. This kit measures total cortisone in serum and plasma and in extracted samples. A cortisone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a white microtiter plate coated with an antibody to capture rabbit antibodies. A cortisone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to cortisone to each well. After a two hour incubation the plate is washed and the chemiluminescent substrate is added. The substrate reacts with the bound cortisone-peroxidase conjugate to produce light. The generated light is detected in a microtiter plate reader capable of reading luminescence. The concentration of the cortisone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Coated White 96 Well Plates 1 or 5 Cortisone Standard 50 uL or 125 uL DetectX® Cortisone CLIA Antibody 3 mL or 13 mL DetectX® Cortisone CLIA Conjugate Concentrate 1 mL or 3.5 mL Conjugate Diluent 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Dissociation Reagent 1 mL or 5 mL Wash Buffer Concentrate 30 mL or 125 mL Substrate Solution A 6 mL or 28 mL Substrate Solution B 6 mL or 28 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated White 96 Well Plates 1 or 5 Coated White 96 Well Plates 1 or 5 Cortisone Standard 50 uL or 125 uL Cortisone Standard 50 uL or 125 uL DetectX® Cortisone CLIA Antibody 3 mL or 13 mL DetectX® Cortisone CLIA Antibody 3 mL or 13 mL DetectX® Cortisone CLIA Conjugate Concentrate 1 mL or 3.5 mL DetectX® Cortisone CLIA Conjugate Concentrate 1 mL or 3.5 mL Conjugate Diluent 3 mL or 13 mL Conjugate Diluent 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Dissociation Reagent 1 mL or 5 mL Dissociation Reagent 1 mL or 5 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL Substrate Solution A 6 mL or 28 mL Substrate Solution A 6 mL or 28 mL Substrate Solution B 6 mL or 28 mL Substrate Solution B 6 mL or 28 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each