Corticosterone Chemiluminescent ELISA Kit

Sample Corticosterone Conc. (pg/mL) %CV 1 819.7 6.6 2 207.7 5.9 3 64.37 11.0 Sample Corticosterone Conc. (pg/mL) %CV Sample Corticosterone Conc. (pg/mL) %CV 1 819.7 6.6 1 819.7 6.6 2 207.7 5.9 2 207.7 5.9 3 64.37 11.0 3 64.37 11.0 Sample Corticosterone Conc. (pg/mL) %CV 1 818.9 9.4 2 199.6 11.3 3 55.6 15.1 Sample Corticosterone Conc. (pg/mL) %CV Sample Corticosterone Conc. (pg/mL) %CV 1 818.9 9.4 1 818.9 9.4 2 199.6 11.3 2 199.6 11.3 3 55.6 15.1 3 55.6 15.1

The DetectX® Corticosterone Immunoassay Kit is designed to quantitatively measure Corticosterone present in extracted dried fecal samples, serum, plasma and tissue culture media samples. Please read the complete kit insert before performing this assay. This kit measures total corticosterone in serum and plasma and in extracted fecal samples. A corticosterone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a white microtiter plate coated with an antibody to capture sheep antibodies. A corticosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a sheep polyclonal antibody to corticosterone to each well. After a two hour incubation the plate is washed and the chemiluminescent substrate is added. The substrate reacts with the bound corticosteroneperoxidase conjugate to produce light. The generated light is detected in a microtiter plate reader capable of reading luminescence. The concentration of the corticosterone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Coated White 96 Well Plates 1 or 5 Each Corticosterone Standard 125 or 625 uL DetectX® Corticosterone CLIA Antibody 3 mL or 13 mL DetectX® Corticosterone CLIA Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Dissociation Reagent 1 mL or 5 mL Wash Buffer Concentrate 30 mL or 125 mL Substrate Solution A 6mL or 28 mL Substrate Solution B 6mL or 28 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated White 96 Well Plates 1 or 5 Each Coated White 96 Well Plates 1 or 5 Each Corticosterone Standard 125 or 625 uL Corticosterone Standard 125 or 625 uL DetectX® Corticosterone CLIA Antibody 3 mL or 13 mL DetectX® Corticosterone CLIA Antibody 3 mL or 13 mL DetectX® Corticosterone CLIA Conjugate 3 mL or 13 mL DetectX® Corticosterone CLIA Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Dissociation Reagent 1 mL or 5 mL Dissociation Reagent 1 mL or 5 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL Substrate Solution A 6mL or 28 mL Substrate Solution A 6mL or 28 mL Substrate Solution B 6mL or 28 mL Substrate Solution B 6mL or 28 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each