Glutathione S-Transferase Fluorescent Kit

Sample GST Activity (mU/mL) %CV 1 315.9 4.6 2 221.2 5.6 3 88.2 4.2 4 22.7 6.6 Sample GST Activity (mU/mL) %CV Sample GST Activity (mU/mL) %CV 1 315.9 4.6 1 315.9 4.6 2 221.2 5.6 2 221.2 5.6 3 88.2 4.2 3 88.2 4.2 4 22.7 6.6 4 22.7 6.6 Sample GST Activity (mU/mL) %CV 1 291.7 12.6 2 218.5 11.0 3 89.6 10.4 4 23.0 15.9 Sample GST Activity (mU/mL) %CV Sample GST Activity (mU/mL) %CV 1 291.7 12.6 1 291.7 12.6 2 218.5 11.0 2 218.5 11.0 3 89.6 10.4 3 89.6 10.4 4 23.0 15.9 4 23.0 15.9

The DetectX® Glutathione S-Transferase Fluorescent Activity Kit is designed to quantitatively measure the activity of GST present in a variety of samples. Please read the complete kit insert before performing this assay. A GST standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. The kit utilizes a non-fluorescent molecule that is a substrate for the GST enzyme which covalently attaches to glutathione (GSH) to yield a highly fluorescent product. Mixing the sample or standard with the supplied Detection Reagent and GSH and incubating at room temperature for 30 minutes yields a fluorescent product which is read at 460 nm in a fluorescent plate reader with excitation at 390 nm. The activity of the GST in the sample is calculated, after making a suitable correction for any dilution of the sample, using software available with most fluorescence plate readers. We have provided a 96 well plate for measurement but this assay is adaptable for higher density plate formats. The end user should ensure that their HTS black plate is suitable for use with these reagents prior to running samples.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Black Half Area 96 well Plate One Plate Glutathione S-Transferase Standard 50 uL GST Detection Reagent 1 vial Dry DMSO 2 mL Assay Buffer 45 mL Glutathione (GSH) 300 uL Component Quantity Component Quantity Black Half Area 96 well Plate One Plate Black Half Area 96 well Plate One Plate Glutathione S-Transferase Standard 50 uL Glutathione S-Transferase Standard 50 uL GST Detection Reagent 1 vial GST Detection Reagent 1 vial Dry DMSO 2 mL Dry DMSO 2 mL Assay Buffer 45 mL Assay Buffer 45 mL Glutathione (GSH) 300 uL Glutathione (GSH) 300 uL