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Formaldehyde Detection Kit

Product Specifications

Reproducibility

Sample Formaldehyde Conc. (uM) %CV 1 9.70 7.3 2 38.3 4.2 3 76.0 3.4 4 162 3.7 Sample Formaldehyde Conc. (uM) %CV Sample Formaldehyde Conc. (uM) %CV 1 9.70 7.3 1 9.70 7.3 2 38.3 4.2 2 38.3 4.2 3 76.0 3.4 3 76.0 3.4 4 162 3.7 4 162 3.7 Sample Formaldehyde Conc. (uM) %CV 1 10.5 6.7 2 36.9 4.5 3 71.1 3.8 4 148.9 4.3 Sample Formaldehyde Conc. (uM) %CV Sample Formaldehyde Conc. (uM) %CV 1 10.5 6.7 1 10.5 6.7 2 36.9 4.5 2 36.9 4.5 3 71.1 3.8 3 71.1 3.8 4 148.9 4.3 4 148.9 4.3

Gene Name

Formaldehyde

Type

Application Kit

Assay Principle

The DetectX® Formaldehyde Kit is designed to quantitatively measure formaldehyde present in tissue culture media and urine samples. Please read the complete kit insert before performing this assay. A formaldehyde standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a black microtiter plate. The fluorescent reaction is initiated with the DetectX® Formaldehyde reagent, which is pipetted into each well. After a short incubation the emission of the generated fluorescent signal is detected in a microtiter plate reader capable of measuring 510 nm fluorescence utilizing 450 nm excitation wavelength. The concentration of the formaldehyde in the sample is calculated, after making a suitable correction for the dilution of the sample, using software available with most fluorescence plate readers.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Human Urine and Tissue Culture Media

Sensitivity

0.715 uM

Reconstitution

2°C to 8°C

Components

Component Quantity Black Half Area 96 Well Plate 2 plates Formaldehyde Standard 500 uL DetectX® Formaldehyde Reagent 5 mL Plate Sealers 2 each Component Quantity Component Quantity Black Half Area 96 Well Plate 2 plates Black Half Area 96 Well Plate 2 plates Formaldehyde Standard 500 uL Formaldehyde Standard 500 uL DetectX® Formaldehyde Reagent 5 mL DetectX® Formaldehyde Reagent 5 mL Plate Sealers 2 each Plate Sealers 2 each

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