What is the catalog number for Peripheral Blood Leukocyte Lysate?

The catalog number for Peripheral Blood Leukocyte Lysate is OCPB00100-100UG.

What sizes are available for Peripheral Blood Leukocyte Lysate?

Peripheral Blood Leukocyte Lysate is available in the following size(s): 0.1 mg.

Is Peripheral Blood Leukocyte Lysate in stock?

Peripheral Blood Leukocyte Lysate is currently made to order / not held in immediate stock. Contact us for lead time.

Peripheral Blood Leukocyte Lysate

Product Specifications

Reactivity

Human

Type

Cell Lysate

Applications

Western blot

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

2 mg/ml

Format

Liquid. Peripheral Blood Leukocyte (PBL's) lysate is supplied in SDS sample buffer containing 50 mM DTT.

Reconstitution

2°C to 8°C|-80°C

Host or Source

Human

Preparation

Peripheral Blood Leukocyte (PBL's) tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors) . Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.