Colon Lysate
Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
Product Specifications
Reactivity
Mouse
Type
Cell Lysate
Source
Mouse colon tissue from balb/c mouse
Applications
Western blot
Assay Protocol
Concentration
2 mg/ml
Format
Liquid. Colon lysate is supplied in SDS sample buffer containing 50 mM DTT.
Reconstitution
2°C to 8°C|-80°C
Host or Source
Mouse
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