What is the catalog number for ALAD Protein Lysate?

The catalog number for ALAD Protein Lysate is OCOA17580-20UG.

What sizes are available for ALAD Protein Lysate?

ALAD Protein Lysate is available in the following size(s): 20 µg.

Is ALAD Protein Lysate in stock?

ALAD Protein Lysate is currently made to order / not held in immediate stock. Contact us for lead time.

ALAD Protein Lysate

Product Specifications

Gene Name

Aminolevulinate dehydratase

Gene Aliases

ALADH; aminolevulinate, delta-, dehydratase; delta-aminolevulinic acid dehydratase; PBGS; porphobilinogen synthase; testicular tissue protein Li 95.

Gene ID

210

Accession Number

https://www.ncbi.nlm.nih.gov/protein/NP_000022

Reactivity

Homo sapiens

Target

Delta-aminolevulinic acid dehydratase, delta-aminolevulinic acid dehydratase isoform b [Homo sapiens], Homo sapiens aminolevulinate dehydratase (ALAD), transcript variant 2, mRNA

Type

Protein Lysate

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Format

Lyophilized

Reconstitution

The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes

Molecular Weight

37.23 kDa

NCBI Gene Symbol

ALAD

Host or Source

HEK293T

Protein Name

A0A140VJL9, NM_000031, NP_000022, P13716, Q6ZMU0

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . To facilitate transportation and protein, the products are supplied as lyophilized proteins.