What is the catalog number for CEACAM18 Protein Lysate?

The catalog number for CEACAM18 Protein Lysate is OCOA15543-20UG.

What sizes are available for CEACAM18 Protein Lysate?

CEACAM18 Protein Lysate is available in the following size(s): 20 µg.

Is CEACAM18 Protein Lysate in stock?

CEACAM18 Protein Lysate is currently made to order / not held in immediate stock. Contact us for lead time.

CEACAM18 Protein Lysate

Product Specifications

Gene Aliases

2010110O04Rik; carcinoembryonic antigen-related cell adhesion molecule 1; carcinoembryonic antigen-related cell adhesion molecule 18; CEA-related cell adhesion molecule 1

Gene ID

729767

Accession Number

NP_001073874

Reactivity

Human

Type

Protein Lysate

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Format

Lyophilized// from 25mM tris HCl pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1x Protein inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4

Reconstitution

The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20° C. Lysate samples are stable for 12 months from date of receipt when stored at -20° C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes._x005F

Molecular Weight

43.9 kDa

NCBI Gene Symbol

CEACAM18

Host or Source

HEK293T

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . To facilitate transportation and protein, the products are supplied as lyophilized proteins.