SPARC Protein Lysate
Product Specifications
Gene Name
Secreted protein acidic and cysteine rich
Gene Aliases
Basement-membrane protein 40; BM-40; OI17; ON; ONT; Osteonectin; Secreted protein acidic and rich in cysteine; secreted protein, acidic, cysteine-rich (osteonectin) ; SPARC.
Gene ID
6678
Accession Number
https://www.ncbi.nlm.nih.gov/protein/NP_003109
Reactivity
Homo sapiens
Target
Homo sapiens secreted protein acidic and cysteine rich (SPARC), transcript variant 1, mRNA, SPARC, SPARC isoform 1 precursor [Homo sapiens]
Type
Protein Lysate
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Format
Lyophilized
Reconstitution
The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
Molecular Weight
34.6 kDa
NCBI Gene Symbol
SPARC
Host or Source
HEK293T
Protein Name
NM_003118, NP_003109, P09486
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Gene Name URL
SPARC
Nucleotide Accession Number
https://www.ncbi.nlm.nih.gov/nuccore/NM_003118
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