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MED27 Protein Lysate

Product Specifications

Gene Name

Mediator complex subunit 27

Gene Aliases

Cofactor required for Sp1 transcriptional activation subunit 8; cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa; CRAP34; CRSP complex subunit 8; CRSP34; CRSP8; epididymis secretory sperm binding protein; MED3; Mediator complex subunit 27; mediator of RNA polymerase II transcription subunit 27; NEDSCAC; p37 TRAP/SMCC/PC2 subunit; transcriptional coactivator CRSP34; TRAP37.

Gene ID

9442

Accession Number

https://www.ncbi.nlm.nih.gov/protein/NP_004260

Reactivity

Homo sapiens

Target

Homo sapiens mediator complex subunit 27 (MED27), transcript variant 1, mRNA, Mediator of RNA polymerase II transcription subunit 27, mediator of RNA polymerase II transcription subunit 27 isoform 1 [Homo sapiens]

Type

Protein Lysate

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Format

Lyophilized

Reconstitution

The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes

Molecular Weight

35.4 kDa

NCBI Gene Symbol

MED27

Host or Source

HEK293T

Protein Name

A0A024R8B7, NM_004269, NP_004260, Q6P2C8

Gene Name URL

MED27

Nucleotide Accession Number

https://www.ncbi.nlm.nih.gov/nuccore/NM_004269

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