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SRPX2 Protein Lysate

Product Specifications

Gene Name

Sushi repeat containing protein X-linked 2

Gene Aliases

BPP; CBPS; PMGX; RESDX; SRPUL; sushi repeat-containing protein SRPX2; sushi-repeat protein upregulated in leukemia; sushi-repeat protein up-regulated in leukemia.

Gene ID

27286

Accession Number

https://www.ncbi.nlm.nih.gov/protein/NP_055282

Reactivity

Homo sapiens

Target

Homo sapiens sushi repeat containing protein X-linked 2 (SRPX2), mRNA, Sushi repeat-containing protein SRPX2, sushi repeat-containing protein SRPX2 precursor [Homo sapiens]

Type

Protein Lysate

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Format

Lyophilized// 25mM Tris-HCl (pH 7.6), 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF & 1mM Na3VO434

Reconstitution

Shipped at room temperature. Upon receiving, store the sample at -20° C. Lysate samples are stable for 12 months from date of receipt when stored at -20° C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Molecular Weight

53 kDa

NCBI Gene Symbol

SRPX2

Host or Source

HEK293T

Protein Name

NM_014467, NP_055282, O60687

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . To facilitate transportation and protein, the products are supplied as lyophilized proteins.

Gene Name URL

SRPX2

Nucleotide Accession Number

https://www.ncbi.nlm.nih.gov/nuccore/NM_014467

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