HBA1 Protein Lysate

Product Specifications

Gene Name

Hemoglobin subunit alpha 1, hemoglobin subunit alpha 2

Gene Aliases

Alpha globin; alpha globin chain; alpha one globin; alpha-2 globin; alpha-2 globin chain; Alpha-globin; delta globin; ECYT7; HBA-T2; HBA-T3; HBH; hemoglobin alpha 1 globin chain; hemoglobin alpha chain; hemoglobin subunit alpha; hemoglobin, alpha 1; hemoglobin, alpha 2; METHBA; mutant hemoglobin alpha 2 globin chain; truncated HbA2.

Gene ID

3039, 3040

Accession Number

https://www.ncbi.nlm.nih.gov/protein/NP_000549

Reactivity

Homo sapiens

Target

Alpha-1-globin, Hemoglobin subunit alpha, hemoglobin subunit alpha [Homo sapiens], Homo sapiens hemoglobin subunit alpha 1 (HBA1), mRNA

Type

Protein Lysate

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Format

Lyophilized

Reconstitution

The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes

Molecular Weight

15.3 kDa

NCBI Gene Symbol

HBA1, HBA2

Host or Source

HEK293T

Protein Name

D1MGQ2, NM_000558, NP_000549, P69905

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . To facilitate transportation and protein, the products are supplied as lyophilized proteins.