PRKAA1 Antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
Protein kinase AMP-activated catalytic subunit alpha 1
Gene Aliases
AMPK, AMPKa1, AMPK alpha 1
Gene ID
5562
Accession Number
NP_006242.5
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant Protein of AMPKalpha 1
Target
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK) . AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Clonality
Monoclonal
Type
Monoclonal Antibody
Applications
WB, IHC
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Reconstitution
Store at -20C. Avoid repeated freeze/thaw cycles.
Fragment
IgG1
Specificity
AMPKalpha 1 Mouse Monoclonal Antibody (9G3) detects endogenous levels of PRKAA1
NCBI Gene Symbol
PRKAA1
Host or Source
Mouse
Protein Name
5'-AMP-activated protein kinase catalytic subunit alpha-1
Gene Name URL
PRKAA1
Nucleotide Accession Number
NM_006251.5
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