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MAPK3 Antibody (Phospho-Tyr222/205)

Product Specifications

CAS Number

9007-83-4

Gene Name

Mitogen-activated protein kinase 3

Gene Aliases

ERK, p38, p40, p41, ERK2, ERT1, NS13, ERK-2, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, p42-MAPK

Gene ID

5594

Accession Number

NP_001035145.1

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic Peptide of Phospho-ERK 1/2 (Y222/205)

Target

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.

Clonality

Monoclonal

Type

Monoclonal Antibody

Applications

IHC

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Reconstitution

Store at -20C. Avoid repeated freeze/thaw cycles.

Fragment

IgG1

Specificity

Phospho-ERK 1/2 (Y222/205) Mouse Monoclonal Antibody (4G3) detects endogenous levels of MAPK1/MAPK3

NCBI Gene Symbol

MAPK3

Host or Source

Mouse

Protein Name

Mitogen-activated protein kinase 3

Gene Name URL

MAPK3

Nucleotide Accession Number

NM_001040056.2

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