AKT1 Antibody (Phospho-Ser473)
Product Specifications
CAS Number
9007-83-4
Gene Name
AKT serine/threonine kinase 1
Gene Aliases
AKT, PKB, RAC, PRKBA, PKB-ALPHA, RAC-ALPHA
Gene ID
207
Accession Number
NP_001014431.1
Reactivity
Human
Immunogen
Synthetic Peptide of Phospho-Akt (S473)
Target
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
Clonality
Monoclonal
Type
Monoclonal Antibody
Applications
WB, IHC
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Reconstitution
Store at -20C. Avoid repeated freeze/thaw cycles.
Fragment
IgG1
Specificity
Phospho-Akt (S473) Mouse Monoclonal Antibody (6F8) detects endogenous levels of AKT1
NCBI Gene Symbol
AKT1
Host or Source
Mouse
Protein Name
RAC-alpha serine/threonine-protein kinase
Gene Name URL
AKT1
Nucleotide Accession Number
NM_001014431.1
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