WEE1 Antibody (Phospho-Ser53)
Product Specifications
CAS Number
9007-83-4
Gene Name
WEE1 G2 checkpoint kinase
Gene Aliases
WEE1A, WEE1hu
Gene ID
7465; 22390; 308937
Reactivity
Human, Mouse, Rat
Immunogen
Synthesized peptide derived from human Wee 1 around the phosphorylation site of S53. Location: 30-110aa.
Target
This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
IHC
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Reconstitution
Store at -20C. Avoid repeated freeze/thaw cycles.
Molecular Weight
72 kDa
Fragment
IgG
Specificity
Phospho-Wee 1 (S53) Polyclonal Antibody detects endogenous levels of Wee 1 protein only when phosphorylated at S53.
NCBI Gene Symbol
WEE1
Host or Source
Rabbit
Gene Name URL
WEE1
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