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RELA Antibody (Phospho-Thr435)

Product Specifications

CAS Number

9007-83-4

Gene Name

V-rel avian reticuloendotheliosis viral oncogene homolog A

Gene Aliases

P65, CMCU, NFKB3

Gene ID

5970; 19697

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Synthesized peptide derived from human NFKappaB-p65 around the phosphorylation site of T435. Location: 370-450aa.

Target

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IHC, IP

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Reconstitution

Store at -20C. Avoid repeated freeze/thaw cycles.

Molecular Weight

60 kDa

Fragment

IgG

Specificity

Phospho-NFKappaB-p65 (T435) Polyclonal Antibody detects endogenous levels of NFKappaB-p65 protein only when phosphorylated at T435.

NCBI Gene Symbol

RELA

Host or Source

Rabbit

Gene Name URL

RELA

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