PPP1R12A Antibody (Phospho-Thr853)

Product Specifications

CAS Number

9007-83-4

Gene Name

Protein phosphatase 1, regulatory subunit 12A

Gene Aliases

MBS, GUBS, M130, MYPT1

Gene ID

4659; 17931; 116670

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived between 590-670aa from human MYPT1 around the phosphorylation site of T853.

Target

MYPT1 is a protein encoded by the PPP1R12A gene which is approximately 115,2 kDa. MYPT1 is localised to the cytoplasm. It is involved in the activation of cAMP-dependent PKA, beta-adrenergic signalling, vascular smooth muscle contraction and actin nucleation by ARP-WASP complex. It regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho and is also a key regulator of protein phosphatase 1C. MYPT1 is expressed in striated muscles, specifically in type 2a fibres. Mutations in the PPP1R12A gene may result in infratentorial cancer and medullomyoblastoma. STJ90822 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous MYPT1 protein which only binds about T853 when T853 is phosphorylated.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

IHC, WB

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol