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MAP2K1 Antibody (Phospho-Ser222/226)

Product Specifications

CAS Number

9007-83-4

Gene Name

Mitogen-activated protein kinase kinase 1

Gene Aliases

MEL, CFC3, MEK1, MKK1, MAPKK1, PRKMK1

Gene ID

5604; 5605; 26395; 26396; 170851; 58960

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human MEK-1/2 around the phosphorylation site of S222/226. Location: 170-250aa.

Target

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IHC

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Reconstitution

Store at -20C. Avoid repeated freeze/thaw cycles.

Molecular Weight

48 kDa

Fragment

IgG

Specificity

Phospho-MEK-1/2 (S222/226) Polyclonal Antibody detects endogenous levels of MEK-1/2 protein only when phosphorylated at S222/226.

NCBI Gene Symbol

MAP2K1, MAP2K2

Host or Source

Rabbit

Gene Name URL

MAP2K1MAP2K2

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