IRS1 Antibody (Phospho-Ser312)
Product Specifications
CAS Number
9007-83-4
Gene Name
Insulin receptor substrate 1
Gene Aliases
HIRS-1
Gene ID
3667; 16367; 25467
Reactivity
Human, Mouse, Rat
Immunogen
Synthesized peptide derived from human IRS-1 around the phosphorylation site of S312. Location: 250-330aa.
Target
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
WB, IF, ELISA
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid// PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Reconstitution
Store at -20° C. Avoid repeated freeze/thaw cycles.
Molecular Weight
170/132 kDa
Fragment
IgG
Specificity
Phospho-IRS-1 (S312) Polyclonal Antibody detects endogenous levels of IRS-1 protein only when phosphorylated at S312.
NCBI Gene Symbol
IRS1
Host or Source
Rabbit
Gene Name URL
IRS1
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