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IRF3 Antibody (Phospho-Ser386)

Product Specifications

CAS Number

9007-83-4

Gene Name

Interferon regulatory factor 3

Gene Aliases

IIAE7

Gene ID

3661

Reactivity

Human

Immunogen

Synthesized peptide derived from human IRF-3 around the phosphorylation site of S386. Location: 330-410aa.

Target

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, ELISA

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid// PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Reconstitution

Store at -20° C. Avoid repeated freeze/thaw cycles.

Molecular Weight

55 kDa

Fragment

IgG

Specificity

Phospho-IRF-3 (S386) Polyclonal Antibody detects endogenous levels of IRF-3 protein only when phosphorylated at S386.

NCBI Gene Symbol

IRF3

Host or Source

Rabbit

Gene Name URL

IRF3

Frequently Asked Questions

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