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NFKBIA Antibody (Phospho-Tyr305)

Product Specifications

CAS Number

9007-83-4

Gene Name

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

Gene Aliases

IKBA, MAD-3, NFKBI, EDAID2

Gene ID

4792; 18035; 25493

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Synthesized peptide derived from human IKappaB- Alpha around the phosphorylation site of Y305. Location: 240-320aa.

Target

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IHC

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Reconstitution

Store at -20C. Avoid repeated freeze/thaw cycles.

Molecular Weight

40 kDa

Fragment

IgG

Specificity

Phospho-IKappaB- Alpha (Tyr305) Polyclonal Antibody detects endogenous levels of IKappaB- Alpha protein only when phosphorylated at Y305.

NCBI Gene Symbol

NFKBIA

Host or Source

Rabbit

Gene Name URL

NFKBIA

Frequently Asked Questions

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