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GAPDH Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Glyceraldehyde-3-phosphate dehydrogenase

Gene Aliases

G3PD, GAPD, HEL-S-162eP

Gene ID

2597; 100042025; 24383

Reactivity

Human, Mouse, Rat, Monkey, Dog, Cow, Hamster, Rabbit, Pig, Sheep, Insect, Yeast

Immunogen

Synthetic Peptide

Target

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) . The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.

Clonality

Monoclonal

Type

Monoclonal Antibody

Applications

WB, IHC

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid. 1 mg/mL in PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Reconstitution

Store at -20C. Avoid repeated freeze/thaw cycles.

Specificity

The antibody detects endogenous GAPDH protein.

NCBI Gene Symbol

GAPDH, GAPDH-PS15

Host or Source

Mouse

Gene Name URL

GAPDHGAPDH-PS15

Frequently Asked Questions

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