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ELK1 Antibody (Phospho-Ser383)

Product Specifications

CAS Number

9007-83-4

Gene Name

ELK1, member of ETS oncogene family

Gene Aliases

ELK1, ETS domain-containing protein Elk-1

Gene ID

2002; 13712

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Elk-1 around the phosphorylation site of S383. Location: 320-400aa.

Target

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IHC, IP

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Reconstitution

Store at -20C. Avoid repeated freeze/thaw cycles.

Molecular Weight

45 kDa

Fragment

IgG

Specificity

Phospho-Elk-1 (S383) Polyclonal Antibody detects endogenous levels of Elk-1 protein only when phosphorylated at S383.

NCBI Gene Symbol

ELK1

Host or Source

Rabbit

Gene Name URL

ELK1

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