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CRKL Antibody (Phospho-Tyr207)

Product Specifications

CAS Number

9007-83-4

Gene Name

V-crk avian sarcoma virus CT10 oncogene homolog-like

Gene Aliases

CRKL, Crk-like protein

Gene ID

1399; 12929; 1.00911e+008

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Synthesized peptide derived from human Crk-L around the phosphorylation site of Y207. Location: 150-230aa.

Target

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IHC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Reconstitution

Store at -20C. Avoid repeated freeze/thaw cycles.

Molecular Weight

39 kDa

Fragment

IgG

Specificity

Phospho-Crk-L (Tyr207) Polyclonal Antibody detects endogenous levels of Crk-L protein only when phosphorylated at Y207.

NCBI Gene Symbol

CRKL, LOC100911000

Host or Source

Rabbit

Gene Name URL

CRKLLOC100911000

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