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CAMK2A Antibody (Phospho-Thr286)

Product Specifications

CAS Number

9007-83-4

Gene Name

Calcium/calmodulin-dependent protein kinase II alpha

Gene Aliases

CAMKA, MRD53, MRT63, CaMKIIalpha, CaMKIINalpha

Gene ID

815; 817; 12322; 108058; 25400; 24246

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human CaMKII Alpha/Delta around the phosphorylation site of T286. Location: 230-310aa.

Target

The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca (2+) /calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM) -dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IHC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Reconstitution

Store at -20C. Avoid repeated freeze/thaw cycles.

Molecular Weight

54 kDa

Fragment

IgG

Specificity

Phospho-CaMKII Alpha/Sigma (T286) Polyclonal Antibody detects endogenous levels of CaMKII Alpha/Sigma protein only when phosphorylated at T286.

NCBI Gene Symbol

CAMK2A, CAMK2D

Host or Source

Rabbit

Gene Name URL

CAMK2ACAMK2D

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