ARAF Antibody (Phospho-Tyr302)
Product Specifications
CAS Number
9007-83-4
Gene Name
A-Raf proto-oncogene, serine/threonine kinase
Gene Aliases
PKS2, A-RAF, ARAF1, RAFA1
Gene ID
369; 11836; 64363
Reactivity
Human, Mouse, Rat
Immunogen
Synthesized peptide derived from human A-Raf around the phosphorylation site of Y302. Location: 250-330aa.
Target
This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
WB, IHC, IF
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Reconstitution
Store at -20C. Avoid repeated freeze/thaw cycles.
Molecular Weight
68 kDa
Fragment
IgG
Specificity
Phospho-A-Raf (Tyr302) Polyclonal Antibody detects endogenous levels of A-Raf protein only when phosphorylated at Y302.
NCBI Gene Symbol
ARAF
Host or Source
Rabbit
Gene Name URL
ARAF
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