EIF4EBP1 Antibody (Phospho-Thr70)
Product Specifications
CAS Number
9007-83-4
Gene Name
Eukaryotic translation initiation factor 4E binding protein 1
Gene Aliases
BP-1, 4EBP1, 4E-BP1, PHAS-I
Gene ID
1978
Reactivity
Human
Immunogen
Synthesized peptide derived from human 4E-BP1 around the phosphorylation site of T70. Location: 10-90aa.
Target
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
IHC
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Reconstitution
Store at -20C. Avoid repeated freeze/thaw cycles.
Molecular Weight
12/35 kDa
Fragment
IgG
Specificity
Phospho-4E-BP1 (T70) Polyclonal Antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at T70.
NCBI Gene Symbol
EIF4EBP1
Host or Source
Rabbit
Gene Name URL
EIF4EBP1
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