ACTB Antibody - N-terminal region

Product Specifications

CAS Number

9007-83-4

Gene Name

Actin, beta

Gene Aliases

BRWS1, PS1TP5BP1

Gene ID

Reactivity

Human, Mouse, Rat, Monkey, Canine

Immunogen

Recombinant peptide derived from within residues 100 aa to the N-terminus of human beta-Actin produced in E. coli.

Target

Actin proteins are highly conserved and ubiquitously expressed in all eukaryotic cells. These proteins are the major components of the cytoskeleton and involved in various types of cell motility.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Polyclonal Antibody

Applications

WB, IF

Purification

Epitope-affinity purified from goat antiserum.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

3 mg/ml

Format

Liquid.// PBS with 20% glycerol and 0.05% sodium azide.

Reconstitution

Store at 2-8C for up to one month. For extended storage, store in -20C freezer. Avoid freeze/thaw cycles.

Molecular Weight

42 kDa

Fragment

IgG

Specificity

Detects a band of approximately 42 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC-1, Jurkat, MNT1, U-118, rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates.