VGLUT1 Antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
Solute carrier family 17 (vesicular glutamate transporter), member 7
Gene Aliases
BNPI, SLC17A1, SLC17A1/VGLUT1, Solute Carrier family 17 member 7, Vesicular glutamate transporter 1, VGLUT 1, Brain-specific Na (+) -dependent inorganic phosphate cotransporter
Gene ID
116638
Accession Number
NP_446311.1
Reactivity
Human, Mouse, Rat
Immunogen
Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1
Target
VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1) . Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K (m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2) .
Clonality
Monoclonal
Clone
S28-9
Conjugation
Unconjugated
Type
Monoclonal Antibody
Applications
WB, IHC
Purification
Protein G Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid.// PBS, pH 7.4 with 50% glycerol and 0.09% sodium azide.
Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Reconstitution
Store at -20° C for 1 year. Avoid repeated freeze/thaw cycles.
Fragment
IgG1
Specificity
Detects ~52kDa. No cross-reactivity against VGlut2.
NCBI Gene Symbol
VGLUT1
Host or Source
Mouse
Gene Name URL
VGLUT1
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