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VGLUT1 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Solute carrier family 17 (vesicular glutamate transporter), member 7

Gene Aliases

BNPI, SLC17A1, SLC17A1/VGLUT1, Solute Carrier family 17 member 7, Vesicular glutamate transporter 1, VGLUT 1, Brain-specific Na (+) -dependent inorganic phosphate cotransporter

Gene ID

116638

Accession Number

NP_446311.1

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1

Target

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1) . Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K (m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2) .

Clonality

Monoclonal

Clone

S28-9

Conjugation

Unconjugated

Type

Monoclonal Antibody

Applications

WB, IHC

Purification

Protein G Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid.// PBS, pH 7.4 with 50% glycerol and 0.09% sodium azide.

Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Reconstitution

Store at -20° C for 1 year. Avoid repeated freeze/thaw cycles.

Fragment

IgG1

Specificity

Detects ~52kDa. No cross-reactivity against VGlut2.

NCBI Gene Symbol

VGLUT1

Host or Source

Mouse

Gene Name URL

VGLUT1

Frequently Asked Questions

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