Hsp25/Hsp27 Antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
Heat shock 27kDa protein 1
Gene Aliases
HSPB1, Heat shock protein beta-1, HSPB1, DMPK-binding protein, MKBP
Gene ID
3315
Accession Number
NP_001532.1
Reactivity
Human, Mouse, Rat, Bovine, Dog, Guinea Pig, Hamster, Sheep
Immunogen
HSP27 peptide
Clonality
Monoclonal
Clone
8A7
Conjugation
Unconjugated
Type
Monoclonal Antibody
Applications
WB, IHC, ICC, IF, IP, FC, FACS
Purification
Protein G Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Dilution
WB (1:5000), ICC/IF (1:200), IHC (1:100) ; optimal dilutions for assays should be determined by the user.
Format
Liquid. In PBS, 50% glycerol.
Buffer
PBS, 50% glycerol, 0.09% sodium azide
Reconstitution
Store at -20C. Shipping with Blue Ice or 4C.
Molecular Weight
25 or 27 kD
Notes
Formerly GWB-D4E959
Fragment
IgG1 Kappa
Specificity
Detects ~25kDa or ~27kDa. Recognizes HSP25 and HSP27, cross reacts with alpha B crystallin.
NCBI Gene Symbol
HSP27
Host or Source
Mouse
Gene Name URL
HSP27
Curated Selection
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