CD163 Antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
Scavenger receptor cysteine-rich type 1 protein M130
Gene Aliases
MOUSE ANTI RAT CD163: Low Endotoxin, ED2
Gene ID
312701
Reactivity
Rat
Immunogen
Rat Spleen cell homogenate.
Target
A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor. Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages. In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only. Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde) .
Clonality
Monoclonal
Clone
ED2
Type
Monoclonal Antibody
Applications
Flow cytometry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot
Purification
Protein A Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid. Phosphate buffered saline
Reconstitution
-20°C
Molecular Weight
175 kDa
Notes
Formerly GWB-Q01273
Fragment
IgG1
Specificity
Mouse anti Rat CD163, clone ED2 recognises the rat ED2 cell surface glycoprotein.
NCBI Gene Symbol
CD163
Host or Source
Mouse
Protein Name
Scavenger receptor cysteine-rich type 1 protein M130
Gene Name URL
CD163
Frequently Asked Questions
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