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CD163 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Scavenger receptor cysteine-rich type 1 protein M130

Gene Aliases

MOUSE ANTI RAT CD163: Low Endotoxin, ED2

Gene ID

312701

Reactivity

Rat

Immunogen

Rat Spleen cell homogenate.

Target

A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor. Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages. In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only. Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde) .

Clonality

Monoclonal

Clone

ED2

Type

Monoclonal Antibody

Applications

Flow cytometry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot

Purification

Protein A Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid. Phosphate buffered saline

Reconstitution

-20°C

Molecular Weight

175 kDa

Notes

Formerly GWB-Q01273

Fragment

IgG1

Specificity

Mouse anti Rat CD163, clone ED2 recognises the rat ED2 cell surface glycoprotein.

NCBI Gene Symbol

CD163

Host or Source

Mouse

Protein Name

Scavenger receptor cysteine-rich type 1 protein M130

Gene Name URL

CD163

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