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Horse IgG (H&L) Antibody - Biotin Conjugated

Product Specifications

CAS Number

9007-83-4

Gene Aliases

Rabbit Anti-Horse IgG Biotin Conjugated Antibody, Rabbit Anti-Horse IgG Biotin Conjugated Antibody

Reactivity

Horse

Immunogen

Horse IgG whole molecule

Target

Anti-Horse IgG Biotin Antibody generated in rabbit detects horse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F (c) region, recognized by high-affinity Fc receptor proteins, as well as the F (ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Clonality

Polyclonal

Conjugation

Biotin

Type

Secondary Antibody

Applications

Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot

Purification

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

2 mg/ml

Format

Lyophilized

Reconstitution

2°C to 8°C|-20°C or -80°C

Molecular Weight

455

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Horse IgG and Horse Serum.

Host or Source

Rabbit

Frequently Asked Questions

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