Horse IgG (H&L) Antibody - Biotin Conjugated
Product Specifications
CAS Number
9007-83-4
Gene Aliases
Rabbit Anti-Horse IgG Biotin Conjugated Antibody, Rabbit Anti-Horse IgG Biotin Conjugated Antibody
Reactivity
Horse
Immunogen
Horse IgG whole molecule
Target
Anti-Horse IgG Biotin Antibody generated in rabbit detects horse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F (c) region, recognized by high-affinity Fc receptor proteins, as well as the F (ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Clonality
Polyclonal
Conjugation
Biotin
Type
Secondary Antibody
Applications
Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
2 mg/ml
Format
Lyophilized
Reconstitution
2°C to 8°C|-20°C or -80°C
Molecular Weight
455
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Horse IgG and Horse Serum.
Host or Source
Rabbit
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