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Sheep IgG (H&L) Antibody - AP Conjugated

Product Specifications

CAS Number

9007-83-4

Gene Aliases

ALP, Alk Phos

Reactivity

Sheep

Immunogen

Sheep IgG whole molecule

Target

Anti-Sheep IgG (H&L) generated in donkey detects sheep Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Sheep IgG is conjugated to alkaline phosphatase.

Clonality

Polyclonal

Conjugation

AP

Type

Secondary Antibody

Applications

Dot blot|Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot

Purification

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid (sterile filtered)

Reconstitution

2°C to 8°C

Molecular Weight

140 kDa

Fragment

IgG

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Sheep IgG and Sheep Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.

Host or Source

Donkey

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