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Rabbit IgG (H&L) Antibody F (ab') 2 - PE Conjugated

Product Specifications

CAS Number

9007-83-4

Reactivity

Rabbit

Immunogen

Fab2 Anti-Rabbit IgG was produced by repeated immunization with Rabbit IgG whole molecule in donkey.

Clonality

Polyclonal

Conjugation

R-PE

Type

Secondary Antibody

Applications

Flow cytometry|Immunofluorescence|Immunohistochemistry|Western blot

Purification

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F (c) or to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Format

Lyophilized

Reconstitution

Store vial at 4C prior to restoration. Restore with 1.0 mL of deionized water (or equivalent) .Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.

Molecular Weight

240000

Host or Source

Donkey

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